Overview
If you’re working with research peptides, knowing how to calculate dosage is essential. Whether you’re working with GLP-1 analogs like Retatrutide or other lyophilized compounds, the process of reconstitution and dose calculation follows a universal logic.
In this guide, you’ll learn:
- How to mix peptides correctly (reconstitution)
- How to convert mg to mL
- How to calculate exact dosages per injection
Step 1: Understand the Basics
When you buy a research peptide, it typically comes as a lyophilized powder (freeze-dried) in milligrams (mg). To dose it, you must first reconstitute it with a sterile solvent, typically bacteriostatic water or sterile saline.
Step 2: The Core Formula
The standard dosage formula is:
Dosage (mg) = (Total mg of peptide / Total mL added) × Injection Volume (mL)
You can rearrange the formula depending on what you want to calculate.
Step 3: Real-World Example
Scenario:
You have 30mg of Retatrutide, and you add 3mL of bacteriostatic water.
This gives you a concentration of:
30mg / 3mL = 10mg per mL
If you want to inject 1mg, you’d draw:
1mg ÷ 10mg/mL = 0.1 mL
This means each 0.1 mL = 1mg of Retatrutide.
Step 4: Creating a Dosage Schedule
If your research calls for 2mg per week, and you’ve mixed it at 10mg/mL, you’ll inject:
2mg ÷ 10mg/mL = 0.2 mL weekly
Track this with a protocol sheet or use our upcoming GLPPlanner Calculator Tool to auto-generate the schedule.
Common Measurement Reference Table
| Desired Dose (mg) | Volume to Inject at 10mg/mL |
|---|---|
| 0.25mg | 0.025 mL |
| 0.5mg | 0.05 mL |
| 1mg | 0.1 mL |
| 2mg | 0.2 mL |
| 3mg | 0.3 mL |
Optional: Using IU (International Units)
Some peptides are measured in IU, especially in the context of growth hormone. For GLP-based peptides, mg to mL is standard, but here’s a reference in case you’re dealing with other compounds:
1mL = 100 IU
If your vial has 5mg and you add 1mL, then:
- 1 IU = 0.05mg
- 10 IU = 0.5mg
Always check the label or supplier’s conversion rate.
FAQs
Q: Can I mix with more or less water?
Yes. More water = easier to dose but less shelf stability. Less water = more concentrated, harder to dose precisely.
Q: How long does reconstituted peptide last?
Generally 2–4 weeks in the refrigerator (2–8°C) if stored properly.
Q: Can I freeze it after mixing?
It’s not recommended for daily-use compounds. Only freeze in aliquots if long-term storage is needed.
Summary
Calculating peptide dosage doesn’t need to be confusing. With a basic formula and a clear understanding of volume and concentration, you can confidently mix and measure research peptides like Retatrutide, Semaglutide, and more.
Use our Peptide Dosage Calculator Tool (coming soon) to automate this process and eliminate human error.
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